Location
Comstock Memorial Union, MSUM
Document Type
Poster
Event Website
https://www.mnstate.edu/sac/
Start Date
18-4-2023 12:00 AM
Publication Date
April 2023
Description
Characterizing phages is becoming increasingly important in biochemistry and biotechnology due to their potential to treat bacterial infections without need for antibiotics. Last year, students attempted to isolate proteins from Microbacterium foliorum infecting phages Babydotz and Rosadiaz. Proteins were successfully extracted; however, after further analysis it was shown that the obtained proteins belonged to the host bacteria and not the phages. The purpose of this study was to find a method to separate phage from host bacterial debris to successfully isolate phage proteins and analyze them later. Isolating phage proteins could help us characterize more phages and know more about their infection mechanisms.
Included in
Biochemistry Commons, Biotechnology Commons, Chemistry Commons
Study of Protein Extraction Techniques for M. foliorum phages
Comstock Memorial Union, MSUM
Characterizing phages is becoming increasingly important in biochemistry and biotechnology due to their potential to treat bacterial infections without need for antibiotics. Last year, students attempted to isolate proteins from Microbacterium foliorum infecting phages Babydotz and Rosadiaz. Proteins were successfully extracted; however, after further analysis it was shown that the obtained proteins belonged to the host bacteria and not the phages. The purpose of this study was to find a method to separate phage from host bacterial debris to successfully isolate phage proteins and analyze them later. Isolating phage proteins could help us characterize more phages and know more about their infection mechanisms.
https://red.mnstate.edu/sac/2023/cshe/3