Location
Comstock Memorial Union, MSUM
Document Type
Presentation
Event Website
https://www.mnstate.edu/sac/
Start Date
4-2022 12:00 AM
Publication Date
4-2022
Description
Studies have configured immunological responses to Aspergillus fumigatus in mice, leading to advanced understandings. However, the comparisons of the immune responses between juvenile (younger than 6 weeks) male and female C57BL/6J mice after A. fumigatus exposure, remains uninvestigated. In this study, we intranasally inoculated 3 week old male and female mice with Aspergillus fumigatus spores dilution of 2 106spores per 40 l. They were exposed to the allergen once a week for 8 consecutive weeks and euthanized on day 28 post the last inoculation. As compared to our last report, we increased the sample size for each group in this study. To compare the immune response, we analyzed bronchoalveolar lavage cell counts to quantify macrophages, lymphocytes, neutrophils, eosinophils, and monocytes after differential staining. We also quantified IgE levels using an ELISA kit to see the allergic response from the repeated exposure of Aspergillus fumigatus. Treatment groups representing both genders have significantly higher lymphocytes. However, macrophages accounted for both the treatment and naive groups have similar quantification.. At the end of the study, our data could indicate that sex differences could be an important factor in shaping the immune response of mice against A. fumigatus which could benefit the design of diagnostics and therapeutics for allergic asthma treatments.
Included in
Animal Diseases Commons, Animal Structures Commons, Cells Commons, Fluids and Secretions Commons, Immune System Diseases Commons, Medical Immunology Commons, Tissues Commons
Variations in antibody response to Aspergillus fumigatus inhalation in mice
Comstock Memorial Union, MSUM
Studies have configured immunological responses to Aspergillus fumigatus in mice, leading to advanced understandings. However, the comparisons of the immune responses between juvenile (younger than 6 weeks) male and female C57BL/6J mice after A. fumigatus exposure, remains uninvestigated. In this study, we intranasally inoculated 3 week old male and female mice with Aspergillus fumigatus spores dilution of 2 106spores per 40 l. They were exposed to the allergen once a week for 8 consecutive weeks and euthanized on day 28 post the last inoculation. As compared to our last report, we increased the sample size for each group in this study. To compare the immune response, we analyzed bronchoalveolar lavage cell counts to quantify macrophages, lymphocytes, neutrophils, eosinophils, and monocytes after differential staining. We also quantified IgE levels using an ELISA kit to see the allergic response from the repeated exposure of Aspergillus fumigatus. Treatment groups representing both genders have significantly higher lymphocytes. However, macrophages accounted for both the treatment and naive groups have similar quantification.. At the end of the study, our data could indicate that sex differences could be an important factor in shaping the immune response of mice against A. fumigatus which could benefit the design of diagnostics and therapeutics for allergic asthma treatments.
https://red.mnstate.edu/sac/2022/cshe/2